UHPLC Analysis of Cannabinoids
Conditions | |
|---|---|
| instrument | Shimadzu Nexera X2 |
| column | Ascentis Express C18, 15 cm x 3.0 mm I.D., 2.7 µm |
| mobile phase | [A] water (0.1% formic acid); [B] acetonitrile (0.085% formic acid) |
| gradient | 70% B to 88% B in 6 min |
| flow rate | 1.0 mL/min |
| pressure | 5075 psi |
| column temp. | 30 °C |
| detector | UV, 220 nm |
| injection | 0.6 µL |
| sample | Mixture of 14 cannabinoids, 0.04 mg/mL, 50:50 water:acetonitrile |
Description | |
|---|---|
| Analysis Note | As the popularity of cannabis-infused products increases, there is a growing need to characterize the type and content of the cannabinoids found in the product. This application demonstrates the ability of the Ascentis Express C18 column to baseline resolve 14 structurally-similar cannabinoids, in under seven minutes, with excellent peak shape. |
| Legal Information | LiChrosolv is a trademark of Merck KGaA, Darmstadt, Germany Ascentis is a trademark of Sigma-Aldrich Co. LLC Snap-N-Spike is a trademark of Cerilliant Corporation Snap-N-Shoot Standards is a trademark of Cerilliant Corporation |
| Application No. | MUR110618A |
Materials
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