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  • Abiotrophia defectiva bleb-associated endophthalmitis confirmed with 16s ribosomal RNA sequencing.

Abiotrophia defectiva bleb-associated endophthalmitis confirmed with 16s ribosomal RNA sequencing.

Journal of glaucoma (2013-08-24)
Ming-Han Hugo Lee, Mitchell Lawlor, Anne J Lee
ABSTRACT

One recognized complication of trabeculectomy with visually devastating potential is blebitis. We present a case of a 74-year-old woman with a culture and polymerase chain reaction-positive Abiotrophia defectiva bleb-associated endophthalmitis. Abiotrophia defectiva is a rare but possible cause of endophthalmitis secondary to blebitis and should be considered in culture-negative cases. Prompt identification, hence directed eradication, of the causative organism in such visually threatening cases may be facilitated by requesting polymerase chain reaction and 16S ribosomal RNA sequencing.

MATERIALS
Product Number
Brand
Product Description

USP
Ceftazidime pentahydrate, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Cephalothin sodium salt
Vancomycin hydrochloride, European Pharmacopoeia (EP) Reference Standard
Cefalotin sodium, European Pharmacopoeia (EP) Reference Standard
Atropine, European Pharmacopoeia (EP) Reference Standard
Ceftazidime, European Pharmacopoeia (EP) Reference Standard
Millipore
Vancomycin supplement, suitable for microbiology
Sigma-Aldrich
Vancomycin hydrochloride from Streptomyces orientalis, ≥85% (Vancomycin B)
Sigma-Aldrich
Vancomycin hydrochloride from Streptomyces orientalis, BioReagent, suitable for plant cell culture
Sigma-Aldrich
Vancomycin hydrochloride from Streptomyces orientalis, ≥900 μg per mg (as vancomycin base)
Sigma-Aldrich
Vancomycin hydrochloride from Streptomyces orientalis, meets USP testing specifications
Atropine for peak identification, European Pharmacopoeia (EP) Reference Standard
Supelco
Atropine solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Ceftazidime for peak identification, European Pharmacopoeia (EP) Reference Standard
Cefalotin for impurity B identification, European Pharmacopoeia (EP) Reference Standard