Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.

Fluorescence resonance energy transfer based immunosensing of human IgG by using quantum dot/GIgG-gold nanoparticles/IgG conjugation.

Journal of nanoscience and nanotechnology (2014-04-18)

Lin Luo, Zhao Liu, Jianjun Li, Jian Zhu

ABSTRACT

A novel immunosensor of human immune globulin (IgG) was fabricated based on the fluorescence transfer between luminescent semiconductor quantum dots (QDs) and gold nanoparticles (AuNPs). AuNPs and CdSe/ZnS QDs were respectively labeled with immune reaction pair:IgG and goat anti-human immunoglobulin (GIgG), by optimizing the conditions including pH value and protein amount. In the assembled QD-GIgG-IgG-AuNP fluorescence resonance energy transfer (FRET) immunocomplex system, the presence of AuNP-IgG directly reduced the fluorescence intensity of the GIgG conjugated QDs. As a result, the concentration of AuNP-IgG had a linear relationship with the fluorescence decrease in a range of 0-1.57 microg/mL. Furthermore, the mechanism of the QDs' fluorescence decay has also been discussed and attributed to the light-induced photobleaching. This novel sensing method achieves quantitative detection of trace proteins, suggesting the potential of biomolecule-AuNPs conjugation based analytical methods in further application.

MATERIALS

Product Number

Brand

Product Description

265829

Sigma-Aldrich

Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

373168

Sigma-Aldrich

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349283

Sigma-Aldrich

Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis

326585

Sigma-Aldrich

Gold, powder, <10 μm, ≥99.9% trace metals basis

326534

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

326526

Sigma-Aldrich

Gold, wire, diam. 0.127 mm, 99.99% trace metals basis

265772

Sigma-Aldrich

Gold, powder, <45 μm, 99.99% trace metals basis

265837

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Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

310980

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

349275

Sigma-Aldrich

Gold, foil, thickness 0.05 mm, 99.99% trace metals basis

349305

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.997% trace metals basis

326542

Sigma-Aldrich

Gold, beads, 1-6 mm, 99.999% trace metals basis

GF37996789

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF47078129

Gold, microleaf disks, 10mm, thinness 0.5μm, specific density 966μg/cm², removable support, 99.99+%

GF73794741

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF22371319

Gold, rod, 25mm, diameter 4.0mm, as drawn, 99.95%

GF05586969

Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%

GF04885937

Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF46990974

Gold, microfoil, disks, 10mm, thinness 0.05μm, specific density 101.3μg/cm², permanent mylar 3.5μm support, 99.99+%

GF25294620

Gold, microfoil, disks, 10mm, thinness 0.01μm, specific density 20.7μg/cm², permanent mylar 3.5μm support, 99.99+%

GF06182940

Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF04839767

Gold, tube, 100mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%

GF43173930

Gold, rod, 25mm, diameter 3.0mm, as drawn, 99.95%

GF39029968

Gold, rod, 100mm, diameter 2.0mm, as drawn, 99.95%

GF59949264

Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF96742479

Gold, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

GF23782350

Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm², permanent mylar 3.5μm support, 99.99+%

GF38598980

Gold, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm², permanent mylar 3.5μm support, 99.99+%

GF26034320

Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%