A373-C6

97011321, human skin, Epithelial-like

• UNSPSC Code: 41106514

Properties

• Product Name: A373-C6, 97011321
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: IL-1 receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human malignant melanoma IL-1 sensitive

Cell Line Description

The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.

Application

IL-1 growth inhibition assay, IL-1 receptor analysis

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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