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SHC003V

Sigma-Aldrich

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

Synonym(s):

MISSION®, MISSION® TurboGFP™ Control Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

Quality Level

100

product line

MISSION®

concentration

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

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General description

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP™ transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP™ (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

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Description
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SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC004V

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles, Contains a gene encoding TagRFP

SHC014V

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA Contains no shRNA insert

Sigma-Aldrich

SHC001

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA

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Articles

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Protocols

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

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