PP0200

GlycoProfile^™ I, In-Gel Deglycosylation Kit

• UNSPSC Code: 12352200
• EC Number: 232-615-7
• NACRES: NA.32

Properties

• shelf life: ≥1 yr at 2‑8 °C
• Quality Level: 200
• storage temp.: 2-8°C

Description

Application

The GlycoProfile^™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Biochem/physiol Actions

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Preparation Note

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Acetonitrile, ≥99.93%, BioSyn^® 50 mL

Kit Components Also Available Separately

• P7367

  PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U

  SDS
• T6567

  Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg

  SDS
• Trypsin Solubilization Reagent

  SDS
• I0408

  Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae

  SDS

Safety Information

• pictograms: GHS02,GHS08,GHS07
• signalword: Danger
• hcodes: H225,H302 + H312 + H332,H315,H319,H334,H335
• pcodes: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 35.6 °F
• flash_point_c: 2.0 °C
• wgk: WGK 3