ABN466

Anti-NANOS2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Nanos homolog 2, NOS-2, NANOS2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• UniProt accession no.: P60321
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NANOS2(339345)

Description

General description

Nanos homolog 2 (NANOS2) is a zinc-finger containing, RNA-binding protein belonging to the nanos family. NANOS2 plays an important role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. It has been implicated in germ cell development in both invertebrates and vertebrates. Unlike NANOS1, whose expression in mice is dispensable, the NANOS2 and NANOS3 proteins are required for germ cell development. NANOS2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while NANOS3-null PGCs are lost during the migration stage regardless of sex.

Specificity

This antibody recognizes the N-terminus of NANOS2.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human NANOS2.

Application

Detect Nanos homolog 2 using this rabbit polyclonal antibody, Anti-NANOS2 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS2 in human brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected NANOS2 in human testis cells.

Quality

Evaluated by Western Blotting in rat brain tissue.

Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS2 in 15 µg of rat brain tissue.

Target description

~15 kDa observed

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable