HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)
CONDITIONS
column
Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase
[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate
0.6 mL/min
pressure
812 psi (56 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
2 μL
sample
Dilute 1:9 in acetonitrile
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany