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Merck
  • A high-performance, non-radioactive potency assay for measuring cytotoxicity: A full substitute of the chromium-release assay targeting the regulatory-compliance objective.

A high-performance, non-radioactive potency assay for measuring cytotoxicity: A full substitute of the chromium-release assay targeting the regulatory-compliance objective.

mAbs (2017-03-11)
Alexis Rossignol, Véronique Bonnaudet, Béatrice Clémenceau, Henri Vié, Laurent Bretaudeau
ABSTRACT

Standardized and biologically relevant potency assays are required by the regulatory authorities for the characterization and quality control of therapeutic antibodies. As critical mechanisms of action (MoA) of antibodies, the antibody-dependent cell-meditated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) must be characterized by appropriate potency assays. The current reference method for measuring cytotoxicity is the

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Sigma-Aldrich
Staurosporine solution from Streptomyces sp., Ready Made Solution, 1 mM in DMSO (100 μg/214 μL), 0.2 μm filtered
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Sodium chromate, 98%
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Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
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Sigma-Aldrich
Trypsin-EDTA solution, 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
Complement sera from guinea pig, lyophilized powder
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
Triton X-100, BioXtra
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
€521.00
Sigma-Aldrich
Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
Triton X-100, laboratory grade
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
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Sigma-Aldrich
Fetal Bovine Serum, non-USA origin, sterile-filtered, suitable for cell culture
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100 μL
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A recombinant, preservative-free antibody is available for your target. Try ZRB1042
€521.00