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  • Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Food chemistry (2014-05-20)
O Kenny, T J Smyth, D Walsh, C T Kelleher, C M Hewage, N P Brunton
ABSTRACT

Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium minus, Taraxacum officinale, Centaurea nigra and Cirsium palustre demonstrated antimicrobial activity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365μg/ml). Ethanol extracts also had higher antioxidant activities and phenolic content demonstrating a link between these compounds and the bioactivity of these extracts. Further investigation into the phenolic content of ethanol extracts using UPLC-MS/MS lead to the identification and quantification of numerous phenolic compounds in all species including; 18 from Cirsium arvense, 16 from Cirsium vulgare, 19 from C. palustre, 15 from C. nigra, 17 from C. scabiosa, 14 from Sonchus asper, 17 from A. minus and 11 from T. officinale.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Capsaicin, natural
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Capsaicin, from Capsicum sp., ≥50% (HPLC)
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Kaempferol, ≥97.0% (HPLC)
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L-Ascorbic acid, tested according to Ph. Eur.
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L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
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2,4,6-Tris(2-pyridyl)-s-triazine, for spectrophotometric det. of Fe, ≥99.0% (HPLC)
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Vanillic acid, purum, ≥97.0% (HPLC)
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L-Ascorbic acid, BioUltra, ≥99.5% (RT)
Supelco
Sodium carbonate, reference material for titrimetry, certified by BAM, >99.5%
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Methanol, analytical standard
Sigma-Aldrich
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
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Sodium carbonate, BioUltra, anhydrous, ≥99.5% (calc. on dry substance, T)
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Vanillic acid, 97%
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L-Ascorbic acid, ACS reagent, ≥99%
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D-(−)-Quinic acid, 98%
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L-Ascorbic acid, 99%
Supelco
L-Ascorbic acid, analytical standard
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Ethanol solution, certified reference material, 2000 μg/mL in methanol
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Vanillic acid, ≥97%, FG
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Rosmarinic acid, 96%
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Apigenin, ≥95.0% (HPLC)
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Sodium carbonate, anhydrous, powder, 99.999% trace metals basis
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(−)-Epicatechin, ≥98% (HPLC), from green tea
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(−)-Epicatechin, ≥90% (HPLC)
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Sodium carbonate, BioXtra, ≥99.0%
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L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
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(−)-Epicatechin gallate, ≥98% (HPLC), from green tea
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L-Ascorbic acid, meets USP testing specifications
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L-Ascorbic acid, reagent grade, crystalline
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Sinapic acid, ≥98%, powder