Skip to Content

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Drug and chemical toxicology (2014-01-08)

Ali Jebali, Seyedhossein Hekmatimoghaddam, Bahram Kazemi, Azra Allaveisie, Alireza Masoudi, Karim Daliri, Najme Sedighi, Javad Ranjbari

ABSTRACT

The purpose of this research was to evaluate toxicity of uncoated magnesium oxide nanoparticles (MgO NPs), MgO NPs coated with Peanut agglutinin (PNA) lectin, and PNA alone on the promastigotes of Leishmania major (L. major) and macrophages of BALB/c mice. On the other hand, antileishmanial property of uncoated MgO NPs, lectin coated MgO NPs, and PNA lectin alone was evaluated, and also macrophage activation was investigated after treatment with these materials by measurement of nitrite, H2O2, and some interleukins. This study showed that PNA lectin and lectin coated MgO NPs had approximately no toxicity on L. major and macrophages, but some toxic effects were observed for uncoated MgO NPs, especially at concentration of 500 µg/mL. Interestingly, lectin coated MgO NPs had the highest antileishmanial activity and macrophage activation, compared with uncoated MgO NPs and PNA lectin.

MATERIALS

Product Number

Brand

Product Description

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

Supelco

Methanol, analytical standard

Supelco

Sulfanilamide, VETRANAL^®, analytical standard

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

Sigma-Aldrich

Isopropyl alcohol, ≥99.7%, FCC, FG

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

Sigma-Aldrich

Sulfanilamide, ≥98%

Sigma-Aldrich

Methanol, NMR reference standard

Supelco

Sulfanilamide melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

USP

Sulfanilamide Melting Point Standard, United States Pharmacopeia (USP) Reference Standard

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

Sigma-Aldrich

Sulfanilamide, puriss. p.a., ≥98% (calc. to the dried substance)

USP

Sulfanilamide, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Hydrogen peroxide solution, 50 wt. % in H₂O, stabilized

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H₂O, meets USP testing specifications

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H₂O, ACS reagent

Sigma-Aldrich

Methanol, Absolute - Acetone free

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sulfanilamide, European Pharmacopoeia (EP) Reference Standard

Supelco

2-Propanol, Pharmaceutical Secondary Standard; Certified Reference Material