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  • Site-specific conjugation of RAFT polymers to proteins via expressed protein ligation.

Site-specific conjugation of RAFT polymers to proteins via expressed protein ligation.

Chemical communications (Cambridge, England) (2013-02-21)
Yan Xia, Shengchang Tang, Bradley D Olsen
ABSTRACT

Site-specific protein conjugates with RAFT polymers were synthesized using expressed protein ligation. Stable micelles were formed from both linear block copolymer and Y-shaped conjugates.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(N-isopropylacrylamide), carboxylic acid terminated, average Mn 10,000
Sigma-Aldrich
Poly(N-isopropylacrylamide), Mn ~40,000
Sigma-Aldrich
Poly(N-isopropyl acrylamide), NHS ester end functionalized, average Mn 5,000