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Merck

The scent of Mycobacterium tuberculosis.

Tuberculosis (Edinburgh, Scotland) (2008-02-26)
Mona Syhre, Stephen T Chambers
PMID18296120
ABSTRACT

Worldwide, tuberculosis (TB) kills nearly 2 million people annually, yet rapid diagnosis still relies on a 100-year-old method of sputum staining for acid-fast bacilli. The advent of solid phase micro-extraction and gas chromatography/mass spectrometry makes it possible to systematically investigate whether volatile metabolites from organisms belonging to the genus Mycobacterium can be used as a rapid and highly selective alternative to the traditional diagnostic methods. We have identified four specific compounds (methyl phenylacetate, methyl p-anisate, methyl nicotinate and o-phenylanisole) from Mycobacterium tuberculosis and Mycobacterium bovis cultures grown in vitro that are distinctive volatile markers. These compounds are detectable before the visual appearance of colonies, potentially useful as the basis of a non-invasive diagnostic test for TB and have characteristic odors.

MATERIALS
Product Number
Brand
Product Description
57551-U
Supelco
SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 4
57286-U
Supelco
SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3
57287-U
Supelco
SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3
57325-U
Supelco
SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3
57320-U
Supelco
SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3
57284-U
Supelco
SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 4
57285-U
Supelco
SPME Fiber Assembly Kit, Assortment #1, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3
57550-U
Supelco
SPME Fiber Assembly Kit, Assortment, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 4
57321-U
Supelco
SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), size 24 ga, Autosampler, pk of 3
57324-U
Supelco
SPME Fiber Assembly Kit, Assortment #4, Fused Silica (1 cm), size 24 ga, Manual Holder, pk of 3
57362-U
Supelco
SPME Fiber Assembly Kit, Assortment #5, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 4
57355-U
Supelco
SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Manual Holder, pk of 3, purple hub
57354-U
Supelco
SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 3, purple hub
57294-U
Supelco
SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hub
57293-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, pink hub
57902-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, pink hub
57291-U
Supelco
SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, green hub
57326-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub
57327-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, pink hub
57333-U
Supelco
SPME Sampling Stand, for use with 4 mL vials
57359-U
Supelco
SPME Portable Field Sampler, coating PDMS/DVB
57342-U
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, red hub
57345-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, blue hub
57357-U
Supelco
SPME Sampling Stand, for use with 15 mL vials
57300-U
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, red hub
57341-U
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, red hub
57310-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub
57346-U
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, blue hub
72420
Sigma-Aldrich
Methyl nicotinate, puriss., ≥99.0% (GC)
M59203
Sigma-Aldrich
Methyl nicotinate, 99%