Skip to Content
Merck
  • Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports (2019-03-21)
Marielle Balzano, Maria De Grandis, Thien-Phong Vu Manh, Lionel Chasson, Florence Bardin, Anne Farina, Arnauld Sergé, Ghislain Bidaut, Pierre Charbord, Léonard Hérault, Anne-Laure Bailly, Amandine Cartier-Michaud, Annie Boned, Marc Dalod, Estelle Duprez, Paul Genever, Mark Coles, Marc Bajenoff, Luc Xerri, Michel Aurrand-Lions, Claudine Schiff, Stéphane J C Mancini
PMID30893599
ABSTRACT

In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1-/- mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.