P2092
Monoclonal Anti-p27Kip1 antibody produced in mouse
clone DCS-72, ascites fluid
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DCS-72, monoclonal
mol wt
antigen 27 kDa
contains
15 mM sodium azide
species reactivity
human, rat, monkey, canine, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:200 using extract of cultured mouse fibroblast line NIH3T3
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDKN1B(1027)
mouse ... Cdkn1b(12576)
rat ... Cdkn1b(83571)
Related Categories
General description
Monoclonal Anti p27Kip1 (mouse IgG1 isotype) is derived from the DCS-72 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant p27Kip1 protein of rodent origin.
Specificity
Using immunoblotting, some preparations may exhibit higher and lower molecular weight bands. which may represent a polyubiquitinated species and breakdown products of p27Kip1.
Immunogen
recombinant rodent p27Kip1 protein.
Application
Monoclonal Anti-p27Kip1 antibody produced in mouse has been used in immunoblotting, immunofluorescence, immunohistochemistry, immunoprecipitation and immunocytochemistry.
Biochem/physiol Actions
p27, the product of the kinase inhibition protein1 (kip1) gene, is an inducible inhibitor of cyclin dependent kinase activity. p27Kip1 interacts strongly with D-type cyclins complexed with CDK4 and more weakly with cyclin E and CDK2 complexes. Regulation by p27Kip1 may be an essential step in the pathway that links mitogenic signals to cell cycle progression and may be a key molecular event in the physiological process of cell cycle commitment or passage through the restriction point. Thus, the amount of p27Kip1 increases in quiescent cells and rapidly decreases after stimulation with specific mitogens. Constitutive expression of p27Kip1 in cultured cells causes cell cycle arrest in G1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Dipak Sapkota et al.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 37(10), 607-615 (2008-08-19)
Several of the S100 gene members have been reported to be differentially expressed in many human pathological conditions, in particular, the malignancies. Identification and quantification of the differentially expressed S100 gene members in oral squamous cell carcinoma (OSCC) might facilitate
Differential Somatostatin Receptor (SSTR) 1-5 Expression and Downstream Effectors in Histologic Subtypes of Growth Hormone Pituitary Tumors3.1
Kiseljak-Vassiliades K, et al.
Molecular and cellular endocrinology, 417(1), 73-83 (2015)
Cellular basis of neuroepithelial bending during mouse spinal neural tube closure
McShane SG, et al.
Developmental Biology, 404(2), 113-124 (2015)
Katja Kiseljak-Vassiliades et al.
Molecular and cellular endocrinology, 417, 73-83 (2015-09-24)
The aim of this study was to examine whether differential expression of somatostatin receptors (SSTR) 1-5 and downstream effectors are different in densely (DG) and sparsely (SG) granulated histological growth hormone (GH) pituitary tumor subtypes. The study included 33 acromegalic
R V Lloyd et al.
The American journal of pathology, 154(2), 313-323 (1999-02-23)
p27kip1 (p27) is a member of the universal cyclin-dependent kinase inhibitor (CDKI) family. p27 expression is regulated by cell contact inhibition and by specific growth factors, such as transforming growth factor (TGF)-beta. Since the cloning of the p27 gene in
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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