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Key Documents

05-369X-555

Sigma-Aldrich

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor™ 555

clone C464.6, Upstate®, from mouse

Synonym(s):

Anti-CMT2DD

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified antibody

antibody product type

primary antibodies

clone

C464.6, monoclonal

species reactivity

mouse, human, rabbit, Xenopus, sheep, monkey, canine, pig, rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_152296.3

UniProt accession no.

Q9N0Z6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATP1A3(478)

Related Categories

Specificity

Specific for the α-1 subunit isoform.

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Application

Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Quality

Immunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40μg of rat brain microsomal preparation.

Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.

Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).

Target description

113 kDa

Physical form

25µg Alexa Fluor™ 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide. Liquid at 4°C.
Protein G Purified

Storage and Stability

Do Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Included Negative Control: Catalog # 16-239, Alexa Fluor™ 555-conjugated Normal Mouse IgG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carles Barceló et al.
Journal of cell science, 126(Pt 20), 4553-4559 (2013-08-15)
Activating mutations in the K-Ras small GTPase are extensively found in human tumors. Although these mutations induce the generation of a constitutively GTP-loaded, active form of K-Ras, phosphorylation at Ser181 within the C-terminal hypervariable region can modulate oncogenic K-Ras function

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