05-665
Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7
clone 8E7, Upstate®, from mouse
Synonym(s):
Anti-Anti-CTNNB, Anti-Anti-EVR7, Anti-Anti-MRD19, Anti-Anti-NEDSDV, Anti-Anti-armadillo
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All Photos(10)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8E7, monoclonal
species reactivity
human, rat, mouse
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)
Related Categories
General description
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as α-catenin and β-catenin. α-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins.
When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC).
Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
When β-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in β-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When β-catenin is not associated with cadherins and α-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC).
Recent evidence suggests that β-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Specificity
Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
The antibody eptiope is 100% conserved in zebrafish.
Immunogen
Epitope: The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag
Application
Immunocytochemistry: This antibody was used in immunocytochemistry and showed positive staining on the membrane and cytosol.
This antibody has also been reported by an independent laboratory to show positive immunostaining for beta-catenin in LiCl-treated 293T cells fixed with methanol (Staal, Frank J. T., 2002).
Flow Cytometry: This antibody was used in flow cytometry at an optimal 1 μg/mL concentration.
Immunohistochemistry: This antibody was used in immunohistochemistry on a colorectal carcinoma tissue array at a 1:300 dilution.
This antibody has also been reported by an independent laboratory to detect beta-catenin in mouse embryo sections (Van Noort, M., 2002).
This antibody has also been reported by an independent laboratory to show positive immunostaining for beta-catenin in LiCl-treated 293T cells fixed with methanol (Staal, Frank J. T., 2002).
Flow Cytometry: This antibody was used in flow cytometry at an optimal 1 μg/mL concentration.
Immunohistochemistry: This antibody was used in immunohistochemistry on a colorectal carcinoma tissue array at a 1:300 dilution.
This antibody has also been reported by an independent laboratory to detect beta-catenin in mouse embryo sections (Van Noort, M., 2002).
Anti-Active-β-Catenin (anti-ABC) Antibody, clone 8E7 is a well characterized Mouse Monoclonal Antibody. This highly published mAb also known as Anti-Catenin beta-1 readily detects beta-Catenin & has been validated in FC, ICC, IHC, IHC(P) & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by immunoblot on RIPA lysates from A431 cells.
Western Blot Analysis: 0.2-2 µg/mL of this antibody detected β-catenin in RIPA lysates from A431 cells.
Western Blot Analysis: 0.2-2 µg/mL of this antibody detected β-catenin in RIPA lysates from A431 cells.
Target description
92 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1k in buffer containing 0.1 M Tris-Glycine, pH 7.4, 15 mM NaCl and 0.05% sodium azide
Storage and Stability
Maintain for 1 year at 2-8°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools.
van Noort, M; Weerkamp, F; Clevers, HC; Staal, FJ
Blood null
Wnt signals are transmitted through N-terminally dephosphorylated beta-catenin.
Staal, Frank J T, et al.
EMBO Reports, 3, 63-68 (2002)
Wild-type BRCA1, but not mutated BRCA1, regulates the expression of the nuclear form of beta-catenin.
Li, H; Sekine, M; Tung, N; Avraham, HK
Molecular Cancer Research null
Canonical Wnt signaling is critical to estrogen-mediated uterine growth
Hou, Xiaonan, et al
Molecular Endocrinology, 18, 3035-3049 (2004)
Atypical molecular profile for joint development in the avian costal joint.
Winslow, BB; Burke, AC
Developmental Dynamics null
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