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  • Differential regulation of macrophage phenotype by mature and pro-nerve growth factor.

Differential regulation of macrophage phenotype by mature and pro-nerve growth factor.

Journal of neuroimmunology (2015-07-23)
Kimberly S Williams, Deirdre A Killebrew, Gillian P Clary, Jaimie A Seawell, Rick B Meeker
ABSTRACT

To characterize the role of neurotrophin receptors on macrophages, we investigated the ability of nerve growth factor (NGF) and its precursor, proNGF, to regulate human macrophage phenotype. The p75 neurotrophin receptor (p75(NTR)) and TrkA were concentrated within overlapping domains on membrane ruffles. NGF stimulation of macrophages increased membrane ruffling, calcium spiking, phagocytosis and growth factor secretion. In contrast, proNGF induced podosome formation, increased migration, suppressed calcium spikes and increased neurotoxin secretion. These results demonstrate opposing roles of NGF and proNGF in macrophage regulation providing new avenues for pharmacological intervention during neuroinflammation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Sortilin Antibody, serum, Chemicon®
Sigma-Aldrich
Anti-p75NTR (Neurotrophin Receptor) Antibody, Upstate®, from rabbit
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Calcein-AM, Small Package (20 X 50 μg ), ≥95.0% (HPLC)
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Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
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Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)
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NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
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Phenylmethanesulfonyl fluoride, ≥99.0% (T)
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Calcein-AM, suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
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Ethidium homodimer, suitable for fluorescence, ~90% (HPCE)
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Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
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Red Blood Cell Lysing Buffer Hybri-Max, liquid, sterile-filtered, suitable for hybridoma