Hydroxyapatite and silk combination-coated dental implants result in superior bone formation in the peri-implant area compared with hydroxyapatite and collagen combination-coated implants.

Hydroxyapatite and silk combination-coated dental implants result in superior bone formation in the peri-implant area compared with hydroxyapatite and collagen combination-coated implants.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (2014-09-23)

HaeYong Kweon, Sang-Woon Lee, Byung-Dong Hahn, Yong-Chan Lee, Seong-Gon Kim

PMID25234528

ABSTRACT

The objective of this study was to compare bone formation after installation of uncoated (UC), hydroxyapatite-coated (HA), collagen plus HA-coated (CH), and silk plus HA-coated (SH) implants. Implants in the UC group had acid-etched surfaces. Surface coating was applied using the aerosol deposition method. Cellular responses on the coated surfaces were examined with scanning electron microscopy. Cellular responses to the surfaces were studied with the corresponding coated discs and MG63 cells. Subsequently, 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alkaline phosphatase (ALP) assays were performed. Peri-implant bone formation was evaluated with the rabbit tibia model. Twenty-four implants from each group were installed. The animals were sacrificed 6 weeks after implant installation. Peri-implant bone formation and implant-to-bone contact were measured in histologic sections. Significance of differences across groups was evaluated using analysis of variance. Scanning electron microscopic images showed that the CH and SH groups exhibited cells that appeared more spread out than those in the other groups. The SH group exhibited the highest value in the MTT assay. The CH group exhibited the highest level of ALP activity. Comparisons of these modifications with the acid-etched surfaces showed that the CH and SH groups displayed significantly greater peri-implant bone formation (P < .001). The SH group displayed significantly greater new bone formation and bone-to-implant contact than did the other groups.

MATERIALS

Product Number

Brand

Product Description

GF24976525

Titanium, rod, 200mm, diameter 25mm, annealed, 99.6+%

GF98611623

Titanium, rod, 200mm, diameter 8.0mm, as drawn, 99.99+%

GF20882998

Titanium, rod, 200mm, diameter 5mm, annealed, 99.6+%

GF49264713

Titanium, tube, 200mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

GF76772391

Titanium, rod, 100mm, diameter 10mm, annealed, 99.6+%

GF69259315

Titanium, rod, 500mm, diameter 6mm, annealed, 99.6+%

GF51690663

Titanium, wire reel, 5m, diameter 0.5mm, as drawn, 99.6+%

GF90688449

Titanium, wire reel, 5m, diameter 0.75mm, as drawn, 99.6+%

GF00558187

Titanium, wire reel, 25m, diameter 1.0mm, as drawn, 99.6+%

GF26694771

Titanium, wire reel, 20m, diameter 0.125mm, annealed, 99.6+%

GF96391402

Titanium, wire reel, 25m, diameter 0.20mm, annealed, 99.6+%

GF46132534

Titanium, wire reel, 100m, diameter 0.25mm, as drawn, 99.6+%

GF76266423

Titanium, wire reel, 0.5m, diameter 1.0mm, annealed, 99.6+%

GF12869062

Titanium, wire reel, 1m, diameter 0.25mm, as drawn, 99.6+%

GF71020235

Titanium, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.6+%

GF16213553

Titanium, wire reel, 10m, diameter 0.125mm, annealed, 99.6+%

GF71783354

Titanium, wire reel, 100m, diameter 0.75mm, as drawn, 99.6+%

H0252

Sigma-Aldrich

Hydroxyapatite, Type I, buffered aqueous suspension

M2128

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, 98%

M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

P8361

Sigma-Aldrich

Phosphatase, Alkaline bovine, recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

P3895

Sigma-Aldrich

Phosphatase, Alkaline from human placenta, ≥10 units/mg solid (in glycine buffer)

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

GF61322699

Titanium, rod, 100mm, diameter 2mm, annealed, 99.6+%

GF66991179

Titanium, rod, 100mm, diameter 2mm, as drawn, 99.99+%

GF16031843

Titanium, mesh, 100x100mm, nominal aperture 0.19mm, wire diameter 0.23mm, 60x60 wires/inch, open area 20%, twill weave

GF13900205

Titanium, tube, 100mm, outside diameter 0.51mm, inside diameter 0.35mm, wall thickness 0.08mm, annealed, 99.6+%

GF65336700

Titanium, rod, 200mm, diameter 2mm, annealed, 99.6+%

GF58567067

Titanium, rod, 100mm, diameter 5mm, annealed, 99.6+%