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[The intensification of the Voges-Proskauer-reaction by fumarate].

Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... (1982-07-01)
R Holländer, J Böhmann, B Grewing
PMID6753419
ABSTRACT

The influence of different substances and growth temperatures on the Voges-Proskauer-reaction of Vibrio spec. (HIM 627-1) was investigated by gas chromatographic analysis of acetoin produced and the Voges-Proskauer-reaction itself. The most efficacious conditions for a maximum production of acetoin and the result of the Voges-Proskauer-reaction, respectively, were cultivation of the bacteria in complex Voges-Proskauer-medium in the presence of 0.03 M glucose and 0.05 M fumarate at 37 degrees C. The examination of various so-called Voges-Proskauer positive bacterial strains showed that the production of acetoin and the Voges-Proskauer-reaction, respectively, was increased by cultivation of the bacteria in the presence of fumarate. Fumarate did not influence the reaction of the so-called Voges-Proskauer negative strains, so that false-positive reactions did not occur.