Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

ABSTRACT

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Acetic acid, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Iron(III) chloride, reagent grade, 97%

Sigma-Aldrich

Eosin Y, Dye content ~99 %

Sigma-Aldrich

Aluminum potassium sulfate dodecahydrate, ACS reagent, ≥98%

Sigma-Aldrich

Hydrochloric acid, ACS reagent, 37%

Sigma-Aldrich

Citric acid, ACS reagent, ≥99.5%

Sigma-Aldrich

Hematoxylin, certified by the Biological Stain Commission

Sigma-Aldrich

Sodium phosphate monobasic, BioXtra, ≥99.0%