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  • Characterization of nanoparticles by matrix assisted laser desorption ionization time-of-flight mass spectrometry.

Characterization of nanoparticles by matrix assisted laser desorption ionization time-of-flight mass spectrometry.

Methods in molecular biology (Clifton, N.J.) (2010-12-01)
Uma Ramalinga, Jeffrey D Clogston, Anil K Patri, John T Simpson
ABSTRACT

Determining the molecular weight of nanoparticles can be challenging. The molecular weight characterization of dendrimers, for example, with varying covalent and noncovalent modifications is critical to their use as therapeutics. As such, we describe in this chapter a protocol for the analysis of these molecules by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
ProteoMass Albumin MALDI-MS Standard, vial of 10 nmol, average mol wt 66,429.09 Da by calculation