- Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
Nature (1989-10-05)
M Sopta, Z F Burton, J Greenblatt
PMID2477704
ABSTRACT
RAP30/74 is a heteromeric general transcription initiation factor which binds to RNA polymerase II. Here we report that preparations of RAP30/74 contain an ATP-dependent DNA helicase whose probable function is to melt the DNA at transcriptional start sites. The sequence of the RAP30 subunit of RAP30/74 indicates that RAP30 may be distantly related to bacterial sigma factors.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
TFIIF, Rap30 subunit, GST tagged human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)