Skip to Content
Merck
All Photos(1)

Documents

T2819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

9.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

Looking for similar products? Visit Product Comparison Guide

General description

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • to neutralize IgG bound to protein A/G column
  • as a component in BigDye buffer for sequencing reaction
  • to block slides

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Genotypic inference of HIV-1 tropism using population-based sequencing of V3
McGovern RA, et al.
Journal of Visualized Experiments, 142(46), e2531-e2531 (2010)
Using improved technology for filter paper-based blood collection to survey wild Sika deer for antibodies to hepatitis E virus
Yu C, et al.
Journal of Virological Methods, 142(1-2), 143-150 (2007)
Experimental protocol for detecting cyanobacteria in liquid and solid samples with an antibody microarray chip
Blanco Y, et al.
Journal of Visualized Experiments, 142(120), e54994-e54994 (2017)
Tris/Tris (textperiodcentered) HCl: a standard buffer for use in the physiologic pH range
Durst RA and Staples BR
Clinical Chemistry, 18(3), 206-208 (1972)
Hao Wang et al.
Plant physiology, 172(2), 1003-1018 (2016-08-18)
Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service