AQ502B
Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed
0.5 mg/mL, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
biotin conjugate
antibody form
purified antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
concentration
0.5 mg/mL
technique(s)
ELISA: suitable
immunofluorescence: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
IgG is the most abundant immunoglobulin and is comprised of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Specificity
Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Immunogen
Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).
Application
Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA & IF.
Immunofluorescence: ≤ 1 μg/106 cells.
ELISA: 1:5,000-1:20,000.
Optimal working dilutions must be determined by the end user.
ELISA: 1:5,000-1:20,000.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Physical form
Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN3.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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