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Merck

STS0200

Sigma-Aldrich

ECO TWEEN® 20

greener alternative

viscous liquid

Szinonimák:

TWEEN® 20, Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
MDL-szám:
UNSPSC kód:
12161902
NACRES:
NA.28

leírás

non-ionic

Minőségi szint

Teszt

≥40%

form

viscous liquid

molekulatömeg

~1228

összetétel

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

környezetbarátabb alternatív termék tulajdonságai

Use of Renewable Feedstocks
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

koncentráció

≥40.0% (GC)

szennyeződések

≤3.0% water

törésmutató

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

mp

98.9 °C

savszám

≤2.2 mg KOH/g

hidroxil érték

96‑108 mg KOH/g

átmeneti hőmérséklet

cloud point 76 °C

oldhatóság

water: 100 mg/mL, clear, faintly yellow to yellow

sűrűség

1.095 g/mL at 25 °C (lit.)

HLB (hidrofil/lipofil egyensúly)

16.7

környezetbarátabb alternatív kategória

SMILES string

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

Nemzetközi kémiai azonosító kulcs

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Általános leírás

ECO TWEEN® 20, also known as Polyoxyethylenesorbitan monolaurate, is a bio-based, nonionic detergent manufactured from a natural fatty acid. This surfactant, derived from natural sources, provides various functional advantages, such as detergency, emulsification, and wetting. Its versatility makes it well-suited for a variety of applications in biochemical and biological research, with common uses including cell lysis, protein extraction, nuclei isolation and cell fractionation.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".

TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Alkalmazás

TWEEN 20 has been used as a component of:
  • washing buffer and blocking buffer in Western blotting
  • blocking buffer in immunohistochemistry
  • washing buffer in ELISA (enzyme linked immunosorbent assay)
  • reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Non-ionic detergent

Tulajdonságok és előnyök

  • 100 % Renewable
  • 100 % Bio-based
  • Certified to the USDA BioPreferred Program
  • Lower carbon footprint than petrochemical-based versions
  • High-purity chemical suitable for a wide variety of research applications

Elkészítési megjegyzés

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Jogi információk

ECO TWEEN is a registered trademark of Croda Inc.
TWEEN is a registered trademark of Croda International PLC

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 1

Lobbanási pont (F)

527.0 °F - Pensky-Martens closed cup

Lobbanási pont (C)

275 °C - Pensky-Martens closed cup


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Dokumentumtár megtekintése

Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruit.
Lannetta PM et al
Physiologia Plantarum, 127.2, 247-259 (2006)
Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle.
Gr?nevik E et al
The journal of gene medicine, 7(2), 218-227 (2005)
Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification.
Seal SE et al
Applied and Environmental Microbiology, 58(11), 3759-3761 (1992)
Jennifer Somerfield et al.
Journal of immunology (Baltimore, Md. : 1950), 185(1), 763-768 (2010-06-04)
Biological therapies, even humanized mAbs, may induce antiglobulin responses that impair efficacy. We tested a novel strategy to induce tolerance to a therapeutic mAb. Twenty patients with relapsing-remitting multiple sclerosis received an initial cycle of alemtuzumab (Campath-1H), up to 120
Human hypoblast formation is not dependent on FGF signalling.
Roode M et al
Developmental Biology, 361(2), 358-363 (2012)

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