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11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

Szinonimák:

affinity matrix, anti-ha

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

UNSPSC kód:
12352203

biológiai forrás

rat

Minőségi szint

100

konjugátum

agarose conjugate

antitest forma

purified immunoglobulin

antitest terméktípus

primary antibodies

klón

3F10, monoclonal

form

slurry

kiszerelés

pkg of 1 mL (settled resin volume)

gyártó/kereskedő neve

Roche

izotípus

IgG1

epitóp szekvencia

YPYDVPDYA

kapacitás

2-8 nmol/mL binding capacity

tárolási hőmérséklet

2-8°C

Általános leírás

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

Egyediség

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Alkalmazás

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of HA-tagged proteins from crude protein extracts

Tulajdonságok és előnyök

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

Minőség

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

Fizikai forma

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

Analízis megjegyzés

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

does not flash

Lobbanási pont (C)

does not flash

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Protocols

Anti-HA Affinity Matrix Protocol & Troubleshooting

Anti-HA Affinity Matrix Protocol & Troubleshooting

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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