MABE1126
Anti-DNA G-quadruplex (G4) Antibody, clone 1H6
clone 1H6, from mouse
Szinonimák:
DNA G-quadruplex (G4)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
1H6, monoclonal
faj reaktivitás (homológia által előrejelzett)
all
technika/technikák
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
izotípus
IgG2bκ
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Általános leírás
Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4). Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an appealing topic in molecular biomedical research.
Egyediség
Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Target DNA structure is not species-specific.
Immunogen
KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
Alkalmazás
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA G-quadruplex (G4) in human pancreas and mouse kidney tissue.
Immunocytochemistry Analysis: A representative lot detected discrete DNA G-quadruplex (G4) foci on the compact chromosome in the metaphase HeLa and murine embryonic stem cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected greatly diminished nuclear DNA G-quadruplex (G4) immunoreactivity upon DNase treatment of paraformaldehyde-fixed and Triton X-100-permeablized HeLa cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected G-quadruplex (G4) stabilizing agent telomestatin/TMS treatment-induced nuclear DNA G4 immunoreactivity in chicken DT40 cells lacking the G4-unwinding helicase FANCJ, while the drug enhancing effect was not seen in human FANCJ-expressing DT40 cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of nuclear DNA G-quadruplex (G4) foci in U20S cells following the treatment of the DNA G4-stabilizing agent telomestatin/TMS (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
ELISA Analysis: Target selectivity of a representative lot was determined by competitive ELISA. Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Flow Cytometry Analysis: A representative lot detected enhanced DNA G-quadruplex (G4) immunoreactivity in HeLa cells upon treatment with the DNA G4-stabilizing agent TMPyP4, Cat. No. 613560 (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunohistochemistry Analysis: A representative lot detected nuclear DNA G-quadruplex (G4) immunoreactivity in various paraffin-embedded human tissue sections, including skin, pancreas, testis, placenta, brain, appendix, colon, and kidney (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected discrete DNA G-quadruplex (G4) foci on the compact chromosome in the metaphase HeLa and murine embryonic stem cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected greatly diminished nuclear DNA G-quadruplex (G4) immunoreactivity upon DNase treatment of paraformaldehyde-fixed and Triton X-100-permeablized HeLa cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected G-quadruplex (G4) stabilizing agent telomestatin/TMS treatment-induced nuclear DNA G4 immunoreactivity in chicken DT40 cells lacking the G4-unwinding helicase FANCJ, while the drug enhancing effect was not seen in human FANCJ-expressing DT40 cells (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunocytochemistry Analysis: A representative lot detected a time-dependent increase of nuclear DNA G-quadruplex (G4) foci in U20S cells following the treatment of the DNA G4-stabilizing agent telomestatin/TMS (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
ELISA Analysis: Target selectivity of a representative lot was determined by competitive ELISA. Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Flow Cytometry Analysis: A representative lot detected enhanced DNA G-quadruplex (G4) immunoreactivity in HeLa cells upon treatment with the DNA G4-stabilizing agent TMPyP4, Cat. No. 613560 (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Immunohistochemistry Analysis: A representative lot detected nuclear DNA G-quadruplex (G4) immunoreactivity in various paraffin-embedded human tissue sections, including skin, pancreas, testis, placenta, brain, appendix, colon, and kidney (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .
Minőség
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: 2.0 µg/mL of this antibody detected DNA G-quadruplex (G4) in HeLa cells.
Immunocytochemistry Analysis: 2.0 µg/mL of this antibody detected DNA G-quadruplex (G4) in HeLa cells.
Fizikai forma
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Yuanfeng Li et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(7), e2103485-e2103485 (2022-01-23)
Diabetic foot ulcers infected with antibiotic-resistant bacteria form a severe complication of diabetes. Antimicrobial-loaded hydrogels are used as a dressing for infected wounds, but the ongoing rise in the number of antimicrobial-resistant infections necessitates new, nonantibiotic based designs. Here, a
Christine M Carle et al.
PLoS genetics, 12(3), e1005842-e1005842 (2016-03-08)
Guanine (G)-rich DNA readily forms four-stranded quadruplexes in vitro, but evidence for their participation in genome regulation is limited. We have identified a quadruplex-binding protein, Lia3, that controls the boundaries of germline-limited, internal eliminated sequences (IESs) of Tetrahymena thermophila. Differentiation
Lauren L Prister et al.
Molecular microbiology, 112(4), 1219-1234 (2019-07-25)
Neisseria gonorrhoeae, the sole causative agent of gonorrhea, constitutively undergoes diversification of the Type IV pilus. Gene conversion occurs between one of the several donor silent copies located in distinct loci and the recipient pilE gene, encoding the major pilin
Xiaolin Wang et al.
iScience, 25(11), 105312-105312 (2022-10-29)
DNA G-quadruplex is a non-canonical secondary structure that could epigenetically regulate gene expression. To investigate the regulating role of G-quadruplex, we devised an integrating method to perform the algorithm profiling and genome-wide analysis for the dynamic change of genomic G-quadruplex
Susanna Stroik et al.
Nucleic acids research, 48(9), 4960-4975 (2020-04-02)
G-quadruplexes represent unique roadblocks to DNA replication, which tends to stall at these secondary structures. Although G-quadruplexes can be found throughout the genome, telomeres, due to their G-richness, are particularly predisposed to forming these structures and thus represent difficult-to-replicate regions.
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