HPLC Analysis of Dansyl-Methionine Enantiomers on Astec® CHIROBIOTIC® R
CONDITIONS
column
CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)
mobile phase
0.1 wt% ammonium trifluoroacetic acid in methanol
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 260 nm
injection
1 μL
sample
5 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC