Skip to Content
Merck
  • Selenium speciation using capillary electrophoresis coupled with modified electrothermal atomic absorption spectrometry after selective extraction with 5-sulfosalicylic acid functionalized magnetic nanoparticles.

Selenium speciation using capillary electrophoresis coupled with modified electrothermal atomic absorption spectrometry after selective extraction with 5-sulfosalicylic acid functionalized magnetic nanoparticles.

Journal of chromatography. A (2015-04-14)
Lizhen Yan, Biyang Deng, Caiying Shen, Chanjuan Long, Qiufen Deng, Chunyao Tao
PMID25865795
ABSTRACT

A new method for selenium speciation in fermented bean curd wastewater and juice was described. This method involved sample extraction with 5-sulfosalicylic acid (SSA)-functionalized silica-coated magnetic nanoparticles (SMNPs), capillary electrophoresis (CE) separation, and online detection with a modified electrothermal atomic absorption spectrometry (ETAAS) system. The modified interface for ETAAS allowed for the introduction of CE effluent directly through the end of the graphite tube. Elimination of the upper injection hole of the graphite tube reduced the loss of the anlayte and enhanced the detection sensitivity. The SSA-SMNPs were synthesized and used to extract trace amounts of selenite [Se(IV)], selenite [Se(VI)], selenomethionine (SeMet), and selenocystine (SeCys2) from dilute samples. The concentration enrichment factors for Se(VI), Se(IV), SeMet, and SeCys2 were 21, 29, 18, and 12, respectively, using the SSA-SMNPs extraction. The limits of detection for Se(VI), Se(IV), SeMet, and SeCys2 were 0.18, 0.17, 0.54, 0.49ngmL(-1), respectively. The RSD values (n=6) of method for intraday were observed between 0.7% and 2.9%. The RSD values of method for interday were less than 3.5%. The linear range of Se(VI) and Se(IV) were in the range of 0.5-200ngmL(-1), and the linear ranges of SeMet and SeCys2 were 2-500 and 2-1000ngmL(-1), respectively. The detection limits of this method were improved by 10 times due to the enrichment by the SSA-SMNP extraction. The contents of Se(VI) and Se(IV) in fermented bean curd wastewater were measured as 3.83 and 2.62ngmL(-1), respectively. The contents of Se(VI), Se(IV), SeMet, and SeCys2 in fermented bean curd juice were determined as 6.39, 4.08, 2.77, and 4.00ngmL(-1), respectively. The recoveries were in the range of 99.14-104.5% and the RSDs (n=6) of recoveries between 0.82% and 3.5%.

MATERIALS
Product Number
Brand
Product Description
HT-SE101
Selenium, pellets, < 5mm, ≥99.999%
209643
Sigma-Aldrich
Selenium, pellets, <5 mm, ≥99.99% trace metals basis
209651
Sigma-Aldrich
Selenium, powder, −100 mesh, ≥99.5% trace metals basis
204307
Sigma-Aldrich
Selenium, pellets, <5 mm particle size, ≥99.999% trace metals basis
229865
Sigma-Aldrich
Selenium, powder, −100 mesh, 99.99% trace metals basis
GF66395212
Selenium, foil, 25x25mm, thickness 3mm, 99.95%
700312
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size, 5 mg/mL in toluene
637106
Sigma-Aldrich
Iron(II,III) oxide, nanopowder, 50-100 nm particle size (SEM), 97% trace metals basis
725366
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 20 nm avg. part. size, 5 mg/mL in H2O
725331
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size, 5 mg/mL in H2O
S8295
Sigma-Aldrich
Sodium selenate, BioXtra
747300
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size (TEM), amine functionalized, 1 mg/mL Fe in H2O, dispersion
747408
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 30 nm avg. part. size (TEM), PEG functionalized, 1 mg/mL Fe in H2O, dispersion
310069
Sigma-Aldrich
Iron(II,III) oxide, powder, <5 μm, 95%
518158
Sigma-Aldrich
Iron(II,III) oxide, 99.99% trace metals basis
725358
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size, 5 mg/mL in H2O
700304
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 20 nm avg. part. size, 5 mg/mL in toluene
700320
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size, 5 mg/mL in toluene
790508
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size (TEM), PEG functionalized, 1 mg/mL Fe in H2O, dispersion
747319
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size (TEM), PEG functionalized, 1 mg/mL Fe in H2O, dispersion
747343
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 5 nm avg. part. size (TEM), amine functionalized, 1 mg/mL Fe in H2O, dispersion
747254
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 10 nm avg. part. size (TEM), carboxylic acid functionalized, 5 mg/mL Fe in H2O, dispersion
747327
Sigma-Aldrich
Iron oxide(II,III), magnetic nanoparticles solution, 30 nm avg. part. size (TEM), amine functionalized, 1 mg/mL Fe in H2O, dispersion