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Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Polyethylenimine derivate conjugated with RGD-TAT-NLS as a novel gene vector.

Bio-medical materials and engineering (2014-09-18)

Wenfang Zhao, Kehai Liu, Shunsheng Chen, Man Man Zhu, Hui Lv, Jing Hu, Yuan Mao

ABSTRACT

To solve the contradiction between the cell toxicity and transfection efficiency of polyethylenimine (PEI) derivate in non-viral gene therapy, a novel gene vector, P123-PEI-R18 was synthesized by using biodegradable PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS. The particle size of P123-PEI-R18/DNA was around 100-250 nm. The gene vector could condense DNA at the weight ratio of 2 and protect plasmid DNA from being dissolved in the blood circulation. Importantly, the complexes exhibited lower cell toxicity and higher transfection efficiency contrasted with PEI 25 kDa in vitro. P123-PEI-R18 holds high potential as a safe and efficient gene vector.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Triethylamine, ≥99.5%

Sigma-Aldrich

Bis(trichloromethyl) carbonate, purum, ≥99.0% (AT)

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Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

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Triethylamine, puriss. p.a., ≥99.5% (GC)

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Triethylamine, BioUltra, ≥99.5% (GC)

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

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Rhein, technical grade

Sigma-Aldrich

Rhein

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Triethylamine, ≥99%

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Triethylamine, ≥99.5%

USP

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Supelco

Triethylamine, analytical standard

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Dichloromethane, Selectophore^™, ≥99.5%

Supelco

Dichloromethane, analytical standard

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Ethyl acetate, analytical standard

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N-Hydroxysuccinimide, purum, ≥97.0% (T)

Supelco

Benzene, analytical standard

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N-Hydroxysuccinimide, 98%

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Benzene, anhydrous, 99.8%

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Triphosgene, reagent grade, 98%

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Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

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4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester, ≥98%, powder

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Ethyl acetate, suitable for HPLC, ≥99.7%

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Dichloromethane, suitable for HPLC, ≥99.8%, contains amylene as stabilizer

Sigma-Aldrich

Ethyl acetate

Sigma-Aldrich

Ethyl acetate

Supelco

Ethyl Acetate, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Ethyl acetate, ACS reagent, ≥99.5%

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, for molecular biology