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Application of the Fenton process in the elimination of helminth eggs.

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)

Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia

ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Iron, powder, −325 mesh, 97%

Iron, IRMM^®, certified reference material, 0.5 mm wire

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

Sigma-Aldrich

Iron, chips, 99.98% trace metals basis

Sigma-Aldrich

Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis

Sigma-Aldrich

Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

Sigma-Aldrich

Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

Iron, rod, 1000mm, diameter 30mm, as drawn, 98+%

Iron, rod, 150mm, diameter 5.0mm, as drawn, 99.99+%

Iron, foil, 100x100mm, thickness 0.15mm, hard, 99.5%

Iron, rod, 200mm, diameter 8.0mm, as drawn, 99.99+%

Iron, foil, light tested, 50x50mm, thickness 0.025mm, hard, 99.5%

Iron, foil, light tested, 25x25mm, thickness 0.0125mm, 99.99+%

Iron, rod, 100mm, diameter 4.8mm, as drawn, 98+%

Iron, foil, not light tested, 50x50mm, thickness 0.002mm, 99.85%

Iron, rod, 100mm, diameter 100mm, as drawn, armcO« soft ingot 99.8%

Iron, rod, 500mm, diameter 19mm, as drawn, soft ingot 99.8%

Iron, foil, light tested, 25x25mm, thickness 0.075mm, hard, 99.5%

Iron, rod, 1000mm, diameter 12.7mm, as drawn, 98+%

Iron, foil, 50mm disks, thickness 0.38mm, hard, 99.5%

Iron, rod, 500mm, diameter 40mm, as drawn, armcO« soft ingot 99.8%

Iron, foil, not light tested, 150x150mm, thickness 0.009mm, 99.85%

Iron, rod, 100mm, diameter 40mm, as drawn, armcO« soft ingot 99.8%

Iron, foil, not light tested, 50x50mm, thickness 0.01mm, 99.99+%