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Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.

Letter: Mass spectrometric approach of high pH- and copper-induced glutathione oxidation.

European journal of mass spectrometry (Chichester, England) (2013-07-12)

Gabi Drochioiu, Laura Ion, Catalina Ciobanu, Laura Habasescu, Ionel Mangalagiu

ABSTRACT

The interaction between copper ions and gamma-L-glutamyl-L-cysteinyl-glycine [glutathione (GSH)] molecules may lead to the formation of the physiologically occurring Cu[I)-[GSH]2 and Cu(II)-GSSG complexes. Since glutathione depletion in neurons and aberrant copper metabolism have been implicated in several neurodegenerative disorders, we studied here the interaction of GSH with copper ions (Cu2+) by electrospray ionization ion trap mass spectrometry (ESI-IT-MS). Besides, we extended this approach to pH in excess of 10 by adding ethanolamine to the solution being investigated. As a result, the ESI-IT-MS spectra revealed novel aspects regarding the speciation of copper-glutathione complex.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ethanolamine, puriss. p.a., ACS reagent, ≥99.0% (GC/NT)

Supelco

Ethanolamine, analytical standard

Sigma-Aldrich

Copper, beads, 2-8 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 1.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, beads, 2-8 mm, 99.9995% trace metals basis

Continuous cast copper (O), BCR^®, certified reference material, rod

Sigma-Aldrich

Ethanolamine, ACS reagent, ≥99.0%

Sigma-Aldrich

Copper, turnings, purum p.a., ≥99.0%

Sigma-Aldrich

Copper, foil, thickness 0.025 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 0.25 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 0.5 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, wire, diam. 1.0 mm, ≥99.9%

Sigma-Aldrich

Copper, wire, diam. 0.64 mm, 99.995% trace metals basis

Sigma-Aldrich

Ethanolamine, purified by redistillation, ≥99.5%

Sigma-Aldrich

Ethanolamine, liquid, BioReagent, suitable for cell culture, ≥98%

Sigma-Aldrich

Ethanolamine, ≥99%

Sigma-Aldrich

Ethanolamine, ≥98%

Sigma-Aldrich

Copper, foil, ≥99.8% (complexometric)

Sigma-Aldrich

Copper, powder, <75 μm, 99%

Sigma-Aldrich

Copper, wire, diam. 2.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, ACS reagent, granular, 10-40 mesh, ≥99.90%

Sigma-Aldrich

Copper, shot, −3-+14 mesh, 99%

Copper (S, P), BCR^®, certified reference material, chips

Sigma-Aldrich

Copper, powder, 99.999% trace metals basis

Copper (O), BCR^®, certified reference material, rod

Sigma-Aldrich

Copper, powder, <425 μm, 99.5% trace metals basis

Sigma-Aldrich

Copper, powder (dendritic), <45 μm, 99.7% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 0.25 mm, 99.98% trace metals basis

Sigma-Aldrich

Copper, powder (spheroidal), 10-25 μm, 98%

Sigma-Aldrich

L-Glutathione oxidized, BioXtra, ≥98%