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  • Comparison of acrylamide and furan concentrations, antioxidant activities, and volatile profiles in cold or hot brew coffees.

Comparison of acrylamide and furan concentrations, antioxidant activities, and volatile profiles in cold or hot brew coffees.

Food science and biotechnology (2020-01-25)
Da-Eun Kang, Haeng-Un Lee, Munkhtugs Davaatseren, Myung-Sub Chung
ABSTRACT

The aim of this study was to investigate the formation of furan and acrylamide and to compare antioxidant capacities and volatile compounds in cold or hot brewed coffees. Cold brews were prepared at 5 °C and 20 °C for 12 h. using steeping and dripping, and hot brews were prepared at 80 °C and 95 °C for 5 min. using the pour-over method. Furan contents of cold steeping at 5 °C and hot brewed at 80 °C showed the higher levels significantly (p < 0.05), which were 17.0 ± 0.5 and 10.6 ± 0.1 ng/mL, respectively. However, acrylamide contents in cold steeping at 5 °C and hot brew at 80 °C showed lower levels, which were 4.1 ± 0.4 and 3.5 ± 0.1 ng/mL respectively. Cold brews at 20 °C showed the highest levels of antioxidant activities while hot brews showed similar levels with cold brews at 5 °C. This study confirms that levels of different beneficial and hazardous chemical compounds could be manipulated by adjusting the coffee extraction conditions.

MATERIALS
Product Number
Brand
Product Description

Supelco
Acrylamide-d3 Standard solution, ~500 mg/L in acetonitrile, analytical standard
Sigma-Aldrich
Furan, ≥99%
Sigma-Aldrich
2,2′-Azobis(2-methylpropionamidine) dihydrochloride, powder or granules, 97%
Sigma-Aldrich
Poly(dimethylsiloxane), viscosity 1.0 cSt (25 °C)
Sigma-Aldrich
Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder
Sigma-Aldrich
Furan-d4, ≥98 atom % D, ≥99% (CP), contains 0.025 wt. % BHT as stabilizer
Sigma-Aldrich
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)
Sigma-Aldrich
Folin & Ciocalteu′s phenol reagent, suitable for determination of total protein by Lowry method, 1.9-2.1 N