Walker rat tumour cell line, which supports the growth of herpes simplex, pseudorabies and vaccinia viruses. The cells are highly tumourigenic in suckling rats.
Culture Medium
Medium 199 + 2mM Glutamine + 5% Horse Serum (HS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C
Other Notes
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