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P5380

Sigma-Aldrich

Protease from Bacillus licheniformis

Type VIII, lyophilized powder, 7-15 units/mg solid

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

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About This Item

CAS Number:
9014-01-1
Enzyme Commission number:
3.4.21.62 (BRENDA, IUBMB)
EC Number:
232-752-2
MDL number:
MFCD00132092
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Bacillus licheniformis

Quality Level

200

type

Type VIII

form

lyophilized powder

specific activity

7-15 units/mg solid

mol wt

27 kDa

purified by

crystallization

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless

foreign activity

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

storage temp.

−20°C

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General description

The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

Non-specific Protease Activity Assay Video
The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tommi Kotila et al.
Nature communications, 9(1), 1892-1892 (2018-05-16)
Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on "re-charging" of ADP-actin monomers with ATP, but whether this reaction requires dedicated proteins in cells, and the underlying mechanism, have remained elusive.
Carley R Benton et al.
The Journal of physiology, 586(6), 1755-1766 (2008-02-02)
Peroxisome proliferator-activated receptors (PPARs) alter the expression of genes involved in regulating lipid metabolism. Rosiglitazone, a PPARgamma agonist, induces tissue-specific effects on lipid metabolism; however, its mode of action in skeletal muscle remains unclear. Since fatty acid translocase (FAT/CD36) was
Marie Deprez et al.
American journal of respiratory and critical care medicine, 202(12), 1636-1645 (2020-07-30)
Rationale: The respiratory tract constitutes an elaborate line of defense that is based on a unique cellular ecosystem.Objectives: We aimed to investigate cell population distributions and transcriptional changes along the airways by using single-cell RNA profiling.Methods: We have explored the
L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE
Sarah E Ehrlicher et al.
Frontiers in endocrinology, 12, 651211-651211 (2021-04-20)
Lipid overload of the mitochondria is linked to the development of insulin resistance in skeletal muscle which may be a contributing factor to the progression of type 2 diabetes during obesity. The targeted degradation of mitochondria through autophagy, termed mitophagy
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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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