Skip to Content
Merck
All Photos(1)

Documents

MABE143

Sigma-Aldrich

Anti-Ago1 Antibody, clone 4G7-E12

clone 4G7-E12, from mouse

Synonym(s):

hAgo1, Argonaute1, eIF-2C 1, eIF2C 1, Q99, GERP95, DKFZp686M13167

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4G7-E12, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_036331

UniProt accession no.

Q9UL18

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AGO1(26523)

General description

Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

Immunogen

GST-tagged recombinant protein corresponding to human Ago1.

Application

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.
Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.

Target description

~97 kDa observed.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Catalina Méndez et al.
Retrovirology, 15(1), 67-67 (2018-10-06)
Current antiretroviral therapy is effective in controlling HIV-1 infection. However, cessation of therapy is associated with rapid return of viremia from the viral reservoir. Eradicating the HIV-1 reservoir has proven difficult with the limited success of latency reactivation strategies and
Roberta Tarallo et al.
Genome biology, 18(1), 189-189 (2017-10-12)
The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts a pivotal role in microRNA maturation and activity and can modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. Estrogen receptor beta (ERβ) is endowed

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service