68724

Fast Green FCF

analytical standard

• Synonym(s): Food green 3
• Linear Formula: C₃₇H₃₄N₂O₁₀S₃Na₂
• CAS Number: 2353-45-9
• Molecular Weight: 808.85
• Colour Index Number: 42053
• Beilstein: 5718212
• EC Number: 219-091-5
• MDL number: MFCD00013053
• UNSPSC Code: 85151701
• PubChem Substance ID: 329761116
• E Number: E143
• NACRES: NA.24

Properties

• grade: analytical standard
• Quality Level: 100
• Assay: ≥98.0% (HPLC)
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• mp: 290 °C (dec.) (lit.)
• application(s): cleaning products; cosmetics; food and beverages; personal care
• format: neat
• SMILES string: [Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
• InChI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• InChI key: RZSYLLSAWYUBPE-UHFFFAOYSA-L

Description

General description

Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H335,H341
• Precautionary Statements: P202 - P261 - P271 - P280 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Muta. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): 352.4 °F - closed cup
• Flash Point(C): 178 °C - closed cup