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MABE327

Sigma-Aldrich

Anti-p53 (pantropic) Antibody, clone DO-1

clone DO-1, from mouse

Synonym(s):

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

General description

Cellular tumor antigen p53 (p53) is a ubiquitous transcription factor that is most widely known for its function as a tumor suppressor, through its regulation of cell growth and survival. p53 is activated in response to cell stress such as DNA damage and induces either cell cycle arrest or apoptosis. It coordinates the expression of multiple genes including p21, GADD45, Bax, and Puma. p53 is regulated by multiple mechanisms: At the post-transcriptional level, the stability of p53 mRNAs is regulated by the binding of molecules such as HuR, Wig-1, RPL26, L26, and WRAP53. Post-translationally, p53 undergoes phosphorylation and acetylation which may also play a role in regulating its activity. It may also undergo ubiquitination via the Akt-MDM2 pathway resulting in degradation by the 20S proteosome. Mutations in p53 are prevalent in many types of cancers, underscoring the therapeutic relevance of this protein.

Specificity

This antibody recognizes the N-terminus of p53 (pantropic).

Immunogen

Epitope: N-terminus
Recombinant protein corresponding to human p53 (pantropic).

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p53 (pantropic) in A431 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal adenocarcinoma tissue and in human prostate cancer tissue.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal cancer cells.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in radiolabelled HOS cell lysate (Vojtesek, B., et al. (1995). 71(6):1253-1256.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in primary breast carcinoma tissue (Beck, T., et al. (1995). Gynecol Oncol. 57(1):96-104.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-p53 (pantropic) Antibody, clone DO-1 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC, IF, IP to detect p53 (pantropic) also known as Cellular tumor antigen p53 or Antigen NY-CO-13.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 (pantropic) in 10 µg of A431 cell lysate. This antibody detects isoforms 1, 2, and 3.

Target description

~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates.

Linkage

Replaces: 04-1083

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oliver I Fregoso et al.
Molecular cell, 50(1), 56-66 (2013-03-13)
Splicing and translation are highly regulated steps of gene expression. Altered expression of proteins involved in these processes can be deleterious. Therefore, the cell has many safeguards against such misregulation. We report that the oncogenic splicing factor SRSF1, which is
A Dey et al.
Cell death and differentiation, 15(2), 263-273 (2007-11-03)
Seliciclib (CYC202, R-Roscovitine) is a 2, 6, 9-substituted purine analog that is currently in phase II clinical trials as an anticancer agent. We show in this study that R-Roscovitine can downregulate nuclear factor-kappa B (NF-kappaB) activation in response to tumor
Alvaro Blanch et al.
PloS one, 8(6), e66436-e66436 (2013-06-27)
The p53 family of transcription factors is a key regulator of cell proliferation and death. In this report we identify the eukaryotic translation elongation factor 1-alpha 1 (eEF1A1) to be a novel p53 and p73 interacting protein. Previous studies have
Arata Shimo et al.
Cancer science, 99(1), 62-70 (2007-10-20)
To elucidate the molecular mechanisms of mammary carcinogenesis and discover novel therapeutic targets for breast cancer, we previously carried out genome-wide expression profile analysis of 81 breast cancer cases by means of cDNA microarray coupled with laser microbeam microdissection of
Kristina Heyne et al.
Nucleic acids research, 38(10), 3159-3171 (2010-02-04)
p63 Is a sequence-specific transcription factor that regulates epithelial stem cell maintenance and epithelial differentiation. In addition, the TAp63 isoform with an N-terminal transactivation domain functions as an inducer of apoptosis during the development of sympathetic neurons. Previous work has

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