Skip to Content
Merck
All Photos(1)

Key Documents

ES009

Millipore

p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)

500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA.

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable

λmax

405 nm

Application

p-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.

This is an excellent substrate for phosphatase based ELISA assays.

It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Physical form

Color Code: Yellow
p-NPP, a single component substrate in proprietary stabilizer.

Storage and Stability

Maintain at 2-8°C for up to one year.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H315,H318,H361fd

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

Serum-free defined cancer stem cell media used to grow and expand cancer cells in 3D tumorsphere aggregates.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service