347420
β-Glucuronidase, Helix pomatia
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
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About This Item
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biological source
Helix pomatia
Quality Level
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
solubility
aqueous buffer: soluble
foreign activity
Aryl sulfatase ≤2%
shipped in
wet ice
storage temp.
2-8°C
Related Categories
General description
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
Biochem/physiol Actions
β-Glucuronidase, a member of the 2 glycosyl hydrolase family, is involved in the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase catalyzes the complete hydrolysis of urinary conjugates. β-Glucuronidase serves as a possible candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. β-glucuronidase-responsive albumin binding prodrugs might be an effective therapeutic practice for treating solid tumors.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will liberate 1.0 µg phenolphthalein from phenolphthalein glucuronide per h at 37°C, pH 5.0.
Physical form
A 65% saturated ammonium sulfate suspension.
Reconstitution
Following reconstitution, store in refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Other Notes
Shibasaki, H., et al. 2001. Steroids66, 795.
Graef, V., et al. 1977. Clin. Chem.23, 532.
Graef, V., et al. 1977. Clin. Chem.23, 532.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Certificates of Analysis (COA)
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Prabha Dwivedi et al.
Chemosphere, 199, 256-262 (2018-02-16)
Human exposure to consumer and personal care products chemicals such as phenols, including parabens and other antimicrobial agents, can be assessed through biomonitoring by quantifying urinary concentrations of the parent chemical or its metabolites, often after hydrolysis of phase II
Isabelle Tranoy-Opalinski et al.
European journal of medicinal chemistry, 74, 302-313 (2014-02-01)
The design of novel antitumor agents allowing the destruction of malignant cells while sparing healthy tissues is one of the major challenges in medicinal chemistry. In this context, the use of non-toxic prodrugs programmed to be selectively activated by beta-glucuronidase
K-C Chen et al.
Cancer gene therapy, 14(2), 187-200 (2006-09-16)
Gene-mediated enzyme prodrug therapy (GDEPT) seeks to increase the therapeutic index of anti-neoplastic agents by promoting selective activation of relatively nontoxic drug derivatives at sites of specific enzyme expression. Glucuronide prodrugs are attractive for GDEPT due to their low toxicity
Loganathan Arul et al.
Bioinformation, 2(8), 339-343 (2008-08-08)
Glycosyl hydrolases hydrolyze the glycosidic bond either in carbohydrates or between carbohydrate and non-carbohydrate moiety. The beta-glucuronidase (beta D-glucuronoside glucuronosohydrolase; EC 3.2.1.31) enzyme belongs to the family-2 glycosyl hydrolase. The E. coli borne beta-glucuronidase gene (uidA) was devised as a
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