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Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate
Upstate®, from mouse, peroxidase conjugate
Synonym(s):
phosphotyrosine
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody product type
primary antibodies
clone
4G10®, monoclonal
PY20, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG2b
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.
Application
Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.
Quality
Routinely evaluated by western blot.
Target description
Varies
Physical form
100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
T Wang et al.
Nihon Rai Gakkai zasshi, 60(3-4), 146-151 (1991-07-01)
A comparative study on the usefulness of Kawatsu's silver impregnating staining method compared with S-100 protein ABC technique for the differential diagnosis of tuberculoid leprosy was carried out. The results of neurohistological examination obtained from both methods were almost the
Mie Andersen et al.
PloS one, 12(6), e0178885-e0178885 (2017-06-02)
Alanine scan of insulin receptor (IR)-B exon 11 and site-directed mutagenesis of amino acid 718 in human IR-A and IR-B were performed. Ligand affinities to wild type and mutated receptors were studied by displacement of radioactive insulin in binding assay
Kogulan Yoganathan et al.
Frontiers in immunology, 13, 848577-848577 (2022-08-23)
The E protein transcription factors E2A and HEB are critical for many developmental processes, including T cell development. We have shown that the Tcf12 locus gives rise to two distinct HEB proteins, with alternative (HEBAlt) and canonical (HEBCan) N-terminal domains
Guillermina M Goñi et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(31), E3177-E3186 (2014-07-23)
Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase (NRTK) with key roles in integrating growth and cell matrix adhesion signals, and FAK is a major driver of invasion and metastasis in cancer. Cell adhesion via integrin receptors is well
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