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Key Documents

14-450-M

Sigma-Aldrich

Cdk1/cyclin B Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

Synonym(s):

Active Cdk1/cyclin B, Cdk1 Protein

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.26

biological source

human

Quality Level

form

liquid

shelf life

6 mo.

mol wt

Mw 35 kDa (cdk1)
Mw 75 kDa (cyclin B)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

NCBI accession no.

UniProt accession no.

storage temp.

−70°C

Gene Information

General description

Research area: Apoptosis
Cyclin dependent kinase 1 (CDK1), a type of serine/threonine kinase, is a catalytic subunit of the M phase-promoting factor (MPF) complex.

Biochem/physiol Actions

Cyclin B protein regulates mitosis in all eukaryotes. Cyclin B binds to cyclin dependent kinase 1 (CDK1), forming a complex that triggers the start of mitosis by phosphorylating specific proteins. Cyclin B regulates the activation, subcellular localization, and target selection of CDK1. Its destruction is essential for the completion of mitosis. Overexpression of human cyclin B1 has been linked to aggressive tumor behavior and is frequently observed in various cancers.
CDK1 plays a key role in driving the M-phase in both meiosis and mitosis. It becomes highly active at the start of the M-phase and becomes inactive at the end. CDK1 controls various important events in the cell, such as DNA replication, segregation, and repair, as well as mRNA transcription and cell morphogenesis. Additionally, it regulates ribosome assembly by targeting specific ribosomal proteins.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of the Histone H1 substrate

Physical form

Purified using Ni2+/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose and Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The crystal structure of human cyclin B
Petri ET, et al.
Cell Cycle, 6(11), 1342-1349 (2007)
Role of cyclin-dependent kinase 1 in translational regulation in the M-phase
Kalous J, et al.
Cells, 9(7), 1568-1568 (2020)
Lin Deng et al.
Molecular cell, 73(5), 915-929 (2019-03-09)
DNA replication errors generate complex chromosomal rearrangements and thereby contribute to tumorigenesis and other human diseases. One mechanism that triggers these errors is mitotic entry before the completion of DNA replication. To address how mitosis might affect DNA replication, we

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