Duolink^® PLA Flow Cytometry Kits

Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink^® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of protein-protein interactions with flow cytometry readouts a reality. With the enhanced sensitivity of the Duolink^® flowPLA Detection Kit, detection of low-abundant protein targets in cell populations is now possible. This easy-to-follow protocol works with any flow cytometry instrument to achieve publication-ready results.

The Duolink^® flowPLA detection kits provide enough material to perform ~40 reactions, each with 100,000 cells in 100 µL reaction volume. Each kit contains the following components:

• Ligation Stock (5X) – Diluted to make 1X Ligation Buffer
• Ligase (1 U/μL) – Added to make the Ligation Solution
• Amplification Stock (5X) – Diluted to make 1X Amplification Buffer
• Polymerase (10 U/μL) – Added to make the Amplification Solution
• Detection Stock (5X) – Diluted to make 1X Detection Buffer

Figure 1. Detection of Post-translational Modifications in Peripheral Blood Mononuclear Cells using Duolink® flowPLA

Figure 2. Detection of Low-abundant Protein-Protein Interactions using Duolink® flowPLA