Poly-lysine Types for Biomedical Applications

George Sitterley

BioFiles 2008, 3.8, 12.

Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components. While some cells are able to synthesize these components, others require an exogenous source, particularly when grown in serum-free culture. To help facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of your cells, We offer a number of sterile, cell culture tested polyamino acids. In histochemical applications both polymers of D- and L-lysine are used to coat slides to promote attachment of cells. Poly-L-lysine has also been reported to improve protein coating of ELISA plates.

We offer both Poly-D-Lysine and Poly-L-Lysine in several molecular weight ranges. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

Poly-L-Lysine is a charge enhancer; therefore, it can be used for coating many surfaces.

Poly-lysines

Name	Form	Molecular Weight	Conc.	Storage Temp.	Application	Cat. No.
Poly-L-lysine	sterile-filtered	70,000-150,000	0.01%	2-8°C	Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.	P4707
Poly-Lornithine	sterile-filtered	sterile-filtered 30,000-70,000	0.01%	2-8°C	Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2.	P4957
Poly-L-lysine	sterile-filtered	150,000-300,000	0.01%	2-8°C	Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.	P4832
Poly-D-lysine	lyophilized powder, γ-irradiated	average mol wt 30,000-70,000	-	−20°C	Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates	P7280
Poly-L-lysine	lyophilized powder, γ-irradiated	30,000-70,000	-	−20°C	Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.	P9155
Poly-D-lysine	lyophilized powder, γ-irradiated	70,000-150,000	-	−20°C	Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates.	P6407
Poly-L-lysine	lyophilized powder, γ-irradiated	70,000-150,000	-	−20°C	Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.	P6282
Poly-D-lysine	lyophilized powder, γ-irradiated	>300,000	-	−20°C	Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates.	P7405
Poly-L-lysine	lyophilized powder, γ-irradiated	≥300,000	-	−20°C	Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.	P5899