- Stable isotope N-phosphoryl amino acids labeling for quantitative profiling of amine-containing metabolites using liquid chromatography mass spectrometry.
Stable isotope N-phosphoryl amino acids labeling for quantitative profiling of amine-containing metabolites using liquid chromatography mass spectrometry.
Analytica chimica acta (2017-06-10)
Shanshan Zhang, Jinwen Shi, Changkai Shan, Chengting Huang, Yile Wu, Rong Ding, Yuhua Xue, Wen Liu, Qiang Zhou, Yufen Zhao, Pengxiang Xu, Xiang Gao
PMID28595723
RESUMEN
Stable isotope chemical labeling liquid chromatography-mass spectrometry (LC-MS) is a powerful strategy for comprehensive metabolomics profiling, which can improve metabolites coverage and quantitative information for exploration of metabolic regulation in complex biological systems. In the current work, a novel stable isotope N-phosphoryl amino acids labeling strategy (SIPAL) has been successful developed for quantitative profiling of amine-containing metabolites in urine based on organic phosphorus chemistry. Two isotopic reagents,