Saltar al contenido
Merck

Methods for preparing fluorescent and neutral red-stained whole mounts of mouse mammary glands.

Journal of mammary gland biology and neoplasia (2009-11-26)
John D Landua, Adriana P Visbal, Michael T Lewis
RESUMEN

Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Neutral Red, for microscopy (Bact., Bot., Fl., Hist., Vit.), indicator (pH 6.8-8.0)
Número de referencia del producto (SKU)
Tamaño de envase
Disponibilidad
Precio
Cantidad