- Effect on separation of injecting samples in a solvent different from the mobile phase.
Effect on separation of injecting samples in a solvent different from the mobile phase.
Journal of chromatography. A (2007-03-03)
Knut Gedicke, Dorota Antos, Andreas Seidel-Morgenstern
PMID17331517
RESUMEN
The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and D,L-threonine as the sample dissolved in pure water. The adsorption isotherms of D,L-threonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data.
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Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
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Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
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Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
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Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 25 cm × 10 mm
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Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Columna quiral para HPLC Astec® CHIROBIOTIC® T, 5 μm particle size, L × I.D. 10 cm × 4.6 mm