- Converting a solvatochromic fluorophore into a protein-based pH indicator for extreme acidity.
Converting a solvatochromic fluorophore into a protein-based pH indicator for extreme acidity.
Angewandte Chemie (International ed. in English) (2012-07-06)
Maiyun Yang, Yanqun Song, Meng Zhang, Shixian Lin, Ziyang Hao, Yuan Liang, Dianmu Zhang, Peng R Chen
PMID22764096
RESUMEN
Live-cell pH measurements: An environment-sensitive fluorophore (green) was site-specifically introduced on HdeA, an acid-resistant chaperone showing pH-mediated conformational changes under low pH conditions. A survey of the attachment sites led to the discovery of one position on HdeA at which the attached fluorophore showed a strong fluorescence increase upon acidification.
MATERIALES