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Deletion analysis of GAL4 defines two transcriptional activating segments.

Cell (1987-03-13)
J Ma, M Ptashne
RESUMEN

We describe the activities of a wide array of deletion mutants of GAL4, a yeast transcriptional activator. We identify two short regions of GAL4, each of which activates transcription when fused to the DNA-binding region of the molecule. Very large portions of GAL4 are not required for gene activation.

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GAL4 [(1-147) - AH] from Saccharomyces cerevisiae, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)